Hypersensitivity (Total 54972 Papers Found)

We have characterized a nuclease hypersensitive chromatin fraction from murine spermatozoa. Endogenous nuclease activity can be induced in mouse epididymal spermatozoa by appropriate stimuli and cause the localized degradation of chromosomal DNA. Based on these observations, we have isolated nuclease hypersensitive chromatin regions released from spermatozoa in the supernatant of pelleted sperm cells, and have cloned and characterized the DNA. Gel electrophoresis of end-labelled released DNA fra ...
There is no immunological mechanism to adequately explain the sudden epidemic in allergies noted in the last 30 years in developed countries. The reduction in the development of allergic disorders observed in individuals infected with parasitic helminths, however, supports a possible role for worms in suppressing allergies. Helminths regulate the immunity of the host to ensure a mutually beneficial environment for the survival of both the parasite and the host. This interplay between helminths a ...
In contrast to the established role of blood vessel remodeling in inflammation, the biologic function of the lymphatic vasculature in acute inflammation has remained less explored. We studied 2 established models of acute cutaneous inflammation, namely, oxazolone-induced delayed-type hypersensitivity reactions and ultraviolet B irradiation, in keratin 14-vascular endothelial growth factor (VEGF)-C and keratin 14-VEGF-D transgenic mice. These mice have an expanded network of cutaneous lymphatic v ...
It has been proposed that infections with helminths can protect from the development of allergic diseases. However, epidemiological and experimental studies have yielded conflicting results. Therefore we investigated if an infection with Nippostrongylus brasiliensis influenced the development of allergen-induced Th2 cell responses in mice. We found a decrease in allergen-induced airway eosinophilia and Eotaxin levels in the airways when mice were infected with the helminths 8 weeks, and especial ...
PURPOSE We examined whether a continuing medical education intervention increased general practitioners' ability to select the proper pharmacological treatment for patients with osteoarthritis. SUBJECTS AND METHODS Eight towns in Quebec, Canada were randomly allocated to one of four intervention options, workshop and decision tree, workshop, decision tree, or no intervention. All general practitioners practicing in each town were eligible to participate. We evaluated all dispensed prescription ...
Random samples are : (1) Allergens are environmental agents which do not satisfy the classical definition of antigens. The protein nature of the active agents in pollen is in doubt. An allergen is identified by its reaction on the body. Many substances form, at .the site of injection, conjugate proteins which are antigens. (2) In skin testing the patient should have discontinued all medication against allergy the evening before the day of testing., although ACTH and cortisone do not interfere si ...
Incubating skeletal muscle fibers with forskolin, an activator of adenylate cyclase, increases the rate at which nicotinic acetylcholine receptors (AChRs) desensitize when exposed to ACh. Several reports indicate that this is due to the phosphorylation of AChRs by cAMP-dependent protein kinase, but other studies suggest that forskolin interacts with AChRs directly and that second-messenger systems are not required. To help clarify this issue, we studied the effects of forskolin and several other ...
BACKGROUND We evaluated the diagnostic usefulness of tuberculin skin testing in the screening for nontuberculous mycobacterial (NTM) infection in children. METHODS We enrolled 180 children who had chronic cervicofacial lymphadenitis in our study. Skin testing was done using antigens of Mycobacterium tuberculosis, Mycobacterium avium, Mycobacterium kansasii, and Mycobacterium scrophulaceum. The reference standard for NTM infection was a positive culture result, identification by PCR, or both. R ...
Concepts—the elementary building-blocks of thought—have long been a focus of research in psychology, and it shows. A naturalistically minded philosopher who goes shopping for a theory of concepts finds herself faced with a fairly dizzying array of options, including definition theories, prototype theories, exemplar theories, theory theories, and an assortment of hybrids. It is with some sense of relief, perhaps, that she learns of a device—what I shall call, following Fodor 1998a, the “s ...
Shellfish allergy is of increasing concern, as its prevalence has risen in recent years. Many advances have been made in allergen characterization. B cell epitopes in the major allergen tropomyosin have been characterized. In addition to tropomyosin, arginine kinase, sarcoplasmic calcium-binding protein, and myosin light chain have recently been reported in shellfish. All are proteins that play a role in muscular contraction. Additional allergens such as hemocyanin have also been described. The ...
series data are sequences of values measured over time. One of the most recent approaches to classification of time series data is to find shapelets within a data set. Time series shapelets are time series subsequences which represent a class. In order to compare two time series sequences using shapelets, existing work uses Euclidean distance measure. But Euclidean distance has following limitation : it requires data to be standardized if scales differ. In this paper, we perform classification o ...
The rejection of sponge matrix allografts across H-2 barriers has generally been found to contain specifically sensitized cytotoxic T cells to donor alloantigen. There is one exception: sponge matrix allografts that differ only with respect to class II alloantigens do not contain specifically sensitized cytotoxic T cells. We therefore investigated the capacity of infiltrating cells removed from sponge matrix allografts to generate delayed hypersensitivity reactions after exposure to fresh alloan ...
Oncostatin M (OnM) signals through cell surface receptors, which utilize the gp130 subunit. In cultured human umbilical vein endothelial cells (HUVEC), OnM transiently elevates mRNA encoding for suppressor of cytokine signaling-3 (SOCS-3). By 1 h of OnM treatment, HUVEC become refractory to the restimulation by OnM, measured as failure to reinduce SOCS-3 mRNA. OnMinduced desensitization also prevents responses to other gp130-signaling cytokines (e.g. leukemia inhibitory factor and interleukin 11 ...
In this in vivo study, the skin reactivity of rabbits to Mineral Trioxide Aggregate (MTA) and Calcium Enriched Mixture (CEM) was compared. Sixteen albino rabbits were used. The dorsal skin in three areas (2 x 2 cm) of the rabbits was shaved 24 h prior to the test. The areas were randomly covered with freshly mixed biomaterials [MTA (n = 16), CEM (n = 16) and normal saline (control; n = 16)], sterile gauze and bandages were placed for 4 h and the biomaterials were washed. The surface areas of the ...
We have developed methods to produce transgenic mice using yeast artificial chromosomes (YACs) and have applied these methods to the analysis of globin gene regulation using 248 kb beta-globin locus YACs (beta-YACs). The advantages of YAC transgenics are: 1) developmental regulation can be studied in the context of the whole locus, 2) mutations may be readily introduced into the YAC, and 3) the effect of these mutations on gene expression can be analyzed. Mice containing the wild-type beta-YAC s ...
There is no approved therapy for food allergies, which affect 12 million people in the United States and millions more worldwide. In the last few years, our research team at Duke has begun to develop protocols to treat peanut and other food allergies. Two distinct therapies are being developed. Oral immunotherapy (OIT), which relies on ingestion of increasing amounts of the allergenic food, has been used to successfully desensitize more than 50 peanut allergic subjects. Sublingual immunotherapy ...
Total joint arthroplasty (TJA) is commonly performed on older adults. Prosthetic joint infection (PJI) is a serious complication of TJA that can significantly impact quality of life and physical function. In this review, we discuss the epidemiology and risk factors for PJIs among older adults. We also offer an overview of current diagnostic, treatment and management strategies for PJI. Given the serious nature of PJI, prevention efforts remain essential. Several approaches to infection preventio ...
BACKGROUND Fasting is a rigorous type of dietary restriction that is associate with a number of health benefits. During fasting, ketone bodies significantly increase in blood and become major body fuels, thereby sparing glucose. In the present study, we investigated effects of fasting on hypersensitivity. In addition, we also investigated the possible role of D-beta-hydroxybutyrate provoked by fasting in the attenuation of immediate hypersensitivity by fasting. METHODS Effects of fasting on sy ...
Es existieren nur wenige Daten zu unerwünschten Arzneiwirkungen (UAW) bei älteren Patienten. Es gilt bei diesen, in der Pharmakotherapie besondere Herausforderungen im Hinblick auf Komorbiditäten, Arzneimittelinteraktionen und Compliance zu berücksichtigen. In dieser Arbeit werden Charakteristika von UAW im Alter betrachtet. Neben Daten aus der Literatur wird über erste Ergebnisse aus dem Leipziger Forschungszentrum für Zivilisationserkrankungen (LIFE) berichtet. Hierbei wurde von 2011 bis ...
Chronic obstructive lung diseases are characterized by the inability to prevent bacterial infection and a gradual loss of lung function caused by recurrent inflammatory responses. In the past decade, numerous studies have demonstrated the importance of nucleotide-mediated bacterial clearance. Their interaction with P2 receptors on airway epithelia provides a rapid ‘on-and-off’ signal stimulating mucus secretion, cilia beating activity and surface hydration. On the other hand, abnormally high ...
The full report is titled: “Effect of breastfeeding on lung function in asthmatic children”. It is in the March–April 2015 issue of Allergy Asthma Proceedings (volume 36, pages 116 to 122). The authors are Hwan Soo Kim, Yoon Hee Kim, Min Jung Kim, Hee Seon Lee, Yoon Ki Han, Kyung Won Kim, Myung Hyun Sohn, and KyuEarn Kim. Breastfeeding and childhood asthma: Does breastfeeding decrease the risk of developing asthma? Asthma and other wheezing disorders are common chronic health illnesses of ...
Contact hypersensitivity has been induced in mice grafted with skin derivatized with dinitrofluorobenzene. At optimal concentration of epicutaneously applied hapten (0.02%), it was found that syngeneic, but not allogeneic, skin grafts induced sensitivity. Genetic identity of graft donors and recipients at class I or class II loci of H-2 was essential and sufficient for induction of sensitization. The fact that Ia antigens on haptenated grafts restricted the response indicates that Langerhans cel ...
Daily treatment with a GnRH agonist (GnRH-A) rapidly inhibits reproductive functions. In order to evaluate the relative importance of desensitization at the pituitary and the gonadal levels, immature male rats were treated daily with the GnRH-A BuserelinR (5 ug/d in oil) starting at 40 days of life. After 1 day treatment, testicular content of LH receptors and responsiveness to LH stimulation were already markedly depressed. After 4 days of treatment, inhibition of testicular functions was compl ...
Dog phobias are common in individuals with autism; however, evidence supporting behavioral interventions is limited. The current study evaluated the efficacy of contact desensitization plus reinforcement on dog phobic behavior exhibited by three children diagnosed with autism. The treatment package improved contact with dogs in analog and naturalistic settings and the improvements were maintained at follow-up and in generalization tests. Parents/caregivers also provided high consumer satisfactio ...
UNLABELLED Approximately fifty percent of stroke survivors have neurological deficits. The evaluation of reaction times permits a study of psychomotor performance, which could be very important for a good rehabilitation outcome. AIM The aim of the study was the evaluation of visual reaction times in a group of patients with cerebrovascular lesions, during inpatient hospitalisation in a rehabilitation centre. MATERIALS AND METHODS 46 right-handed patients (34M, 12F) with unilateral cerebrovas ...
Poly(DTH succinate) is a new, degradable polymer with potential applications as a medical implant material. This polymer can be classified as an alternating copolymer of succinic acid and desaminotyrosyl-tyrosine hexyl ester (DTH), a diphenolic monomer derived from the natural amino acid L-tyrosine. In this study, the effects of water uptake (hydration) on the secondary relaxations of poly(DTH succinate) were investigated using the technique of thermally stimulated depolarization currents (TDSC) ...
OBJECTIVE Flow cytometric quantification of in vitro basophil activation can be quite performant and reliable tool to measure IgE-dependent allergen-specific responses in allergic patients. Current study aimed to evaluate the clinical relevance of basophil activation test (BAT) for the diagnosis of pediatric grass pollen and house dust mite (HDM) allergies. METHODS Forty-seven patients suffering from allergic rhinitis with HDM and grass pollen co-sensitization with clinical history of allergi ...
Cytochrome P450 aromatase is a key steroidogenic enzyme that converts androgens to estrogens in vertebrates. There is much interest in aromatase inhibitors (AIs) both because of their use as pharmaceuticals in the treatment of estrogen-sensitive breast cancers, and because a number of environmental contaminants can act as AIs, thereby disrupting endocrine function in humans and wildlife through suppression of circulating estrogen levels. The goal of the current work was to develop a mechanism-ba ...
The Computer network is a heart of the communication. lots of things individual can do on the internet such as email, credit card transaction, net banking, online shopping. Due to this increasing dependency of internet we have to significantly improve the security of internet. Hence, there is an instant need to recognize and detect the attacks. Intrusion Detection is defined as a process of diagnosing the attack and the sign of malicious activity in a computer network by evaluating the system co ...
Neutrophil hyperfunction of Behçet's disease is in part regulated by genetical factors, especially related to HLA-B51 genes and by immunological abnormalities. Regarding the latter points, Behçet's disease worsens with abnormal regulation by gamma delta T cells and alpha beta T cells. Indeed, our own studies and those of other laboratories suggest that human heat shock protein may be one of the triggering factors for activation of both the gamma delta T cells and alpha beta T cells. These acti ...
Peanut is a rich source of plant protein which is inexpensive and abundant in nature. The peanut proteins however cause hypersensitive immunogenic responses in certain individuals. A minute amount of contamination may cause strong allergic reactions and even death. Many chemical pretreatment procedures have been developed and prescribed earlier for removal of this allergenicity. In the present article we have observed trace level Fe(III) and Cu(II) complexation of the protein fractions of peanut ...
Infection with respiratory syncytial virus (RSV) in neonatal mice causes more aggressive airway disease when the mice are reinfected with the same virus as adults. However, the underlying mechanisms responsible for this phenomenon are not entirely defined. Natural helper (NH) cells are considered a key factor for virus-induced or exacerbated airway inflammation and airway hyper-responsiveness by producing type 2 cytokines. To confirm whether NH cells are involved in the aggravated lung pathology ...
Repair under non-growth conditions of DNA double-strand breaks (DSB) and chromatin sites sensitive to S1 endonuclease (SSS) induced by 60Cobalt-gamma rays were monitored in repair-competent and deficient strains of Saccharomyces cerevisiae by pulsed field gelelectrophoresis. In stationary-phase cells of a repair-competent RAD diploid, and an excision-deficient rad3-2 diploid, SSS are repaired as efficiently as DSB, whereas in a repair-competent RAD haploid, and a rad 50-1 diploid, neither SSS no ...
SK&F 78729-A, one of a series of pyranenamine compounds, was studied in several laboratory models of immediate-type hypersensitivity reactions. In vivo, SK&F 78729-A, i.v./p.o., inhibited the antigen-induced rat 48-hr passive cutaneous anaphylaxis by a mechanism other than end-organ antagonism to either histamine or serotonin. In vitro, the compound produced a concentration-related inhibition of the immunologic release of histamine and slow-reacting substance of anaphylaxis from passively sensit ...
Recently, mutations in the zinc finger MYND-type containing 11 (ZMYND11) gene were identified in patients with autism spectrum disorders, intellectual disability, aggression, and complex neuropsychiatric features, supporting that this gene is implicated in 10p15.3 microdeletion syndrome. We report a novel de novo variant in the ZMYND11 gene (p.Ser421Asn) in a patient with a complex neurodevelopmental phenotype. The patient is a 24-yr-old Caucasian/Filipino female with seizures, global developmen ...
BACKGROUND A multidrug regimen including isoniazid, rifampicin, pyrazinamide and ethambutol is commonly used as first-line treatment for tuberculosis. However, this regimen can occasionally result in severe adverse drug reactions, such as drug reaction with eosinophilia and systemic symptoms (DRESS) syndrome and drug-induced liver injury. The culprit drug and mechanistic basis for the hypersensitive reaction are unknown. OBJECTIVES To investigate drug-specific T-cell responses in patients with ...
Aspirin desensitization is a valuable treatment for aspirin-sensitive sinusitis. We present a case where long-term desensitization failed. While undergoing desensitization, our patient had prolonged severe asthmatic reactions and therefore received high intravenous doses of prednisone. We hypothesize that high steroid doses administered at the time of desensitization may have raised the threshold of intolerance to a point where the administered aspirin doses were tolerated. Consequently, symptom ...
An important cloud computing is a new generation of computing and is based on virtualization technology. More and more applications are being deployed in cloud environments. Malware detection or antivirus software has been recently provided as a service in the cloud. A cloud antivirus provider hosts a number of virtual machines each running the same or different antivirus engines on potentially different sets of workloads (files). From the provider's perspective, the problem of optimally allocat ...
The prevalence of tuberculous infection in residents of a large nursing facility in New York City was assessed. The population was predominantly female (80%) and white (86%), with almost one-half (49%) being foreign born. The mean age of the group was 86.1 years. Four hundred and fifty-five residents were given tuberculin skin tests using the two-step Mantoux test procedure. The prevalence of tuberculous infection was 27.5% with only slight differences being noted between the native-born (28.8%) ...
Decision analysis is a tool that clinicians can use to choose an option that maximizes the overall net benefit to a patient. It is an explicit, quantitative, and systematic approach to decision making under conditions of uncertainty. In this article, we present two teaching tips aimed at helping clinical learners understand the use and relevance of decision analysis. The first tip demonstrates the structure of a decision tree. With this tree, a clinician may identify the optimal choice among com ...
BALB/c mice injected intradermally with 10(5) or higher doses of formaldehyde-fixed promastigotes (FFP) of Leishmania major developed strong delayed-type hypersensitivity (DTH) to leishmanial antigens injected into the hind footpad 3 to 10 days later. The DTH peaked 15 to 18 h after footpad injection and disappeared by 48 h. This specific DTH correlated with the homing of 51Cr-labeled syngeneic bone marrow cells and the infiltration of proliferating cells to the site of antigen administration. S ...
The beta-fraction of the venom of the solitary wasp Philanthus triangulum inhibited glutamate-induced potentials in muscle fibres of the locust in a dose-dependent manner (50% block at 2 micrograms/ml). Single channel behaviour and miniature excitatory postsynaptic currents were not affected. The blocking effect disappeared after treatment of the muscle with the lectin concanavalin A which prevents desensitization of glutamate receptors. Block of potentials induced by iontophoretic application o ...